Not known Facts About attune flow cytometer
Not known Facts About attune flow cytometer
Blog Article
Knowledge-enriched characterization of mobile populations. Morphological data from photographs can incorporate on the richness of flow cytometry facts. By way of example, the figure reveals an if not standard apoptosis assay utilizing Annexin V and PI, including mobile imaging to characterize cells in Each and every populace to reveal morphologically distinct attributes. These insights could not have been received from multiplex staining by itself.
As samples are obtained over the Attune CytPix Flow Cytometer, the high-pace brightfield camera captures and outlets photographs of detected occasions in a rate around six,000 photos per second, depending on the flow level and image dimension. For larger overall flexibility, Attune Cytometric Software package permits you to adjust the impression capture frequency as needed and choose a specific gate for graphic seize, if needed.
Blend acoustic focusing technologies with substantial-parameter Examination for flexible spectral unmixing and traditional workflows.
Uncover new insights into your experimental data by visualizing populations making use of combined fluorescence and morphology information. Our program interface wizards enable downsampling, concatenation (merged documents) and dimensionality equipment which might be built in to the software package for an intuitive, streamlined details Assessment experience.
*Pricing is indicative only which is matter to change. Please Get hold of your Agilent consultant with any questions. The information set forth herein just isn't an offer or simply a dedication, both equally of that are subject to credit rating approval, conditions and terms, and availability with Agilent. Agilent Money Solutions is on the market in chosen international locations.
Listed here, a highly skilled consumer has gated singlets confidently. Just after evaluating the guide singlet gate, the CytPix image-derived parameter ParticleCount reveals this gate incorporates over 4% aggregates.
Optimizing gating system. Even strong manual singlet gating is mistake-vulnerable and remains a subjective choice position in Nearly all flow cytometry assays. Imaging may be used to verify and adjust gates to include only solitary cells of interest.
Our researchers will structure panels for you as many times you require—gratis, no product buy essential.
Early indications of this contamination experienced initially been dismissed as debris. Backgating to discover new populations. Backgating imaged cells within the Attune CytPix Flow Cytometer also helps you to use morphological characteristics to discover intriguing subpopulations that would not be apparent from flow cytometry details alone.
Users can view function visuals in real-time to confirm sample excellent, optimize protocols, and file the morphology get more info of every mobile inhabitants determined for Examination. Imaging capture also Gains from acoustic focusing, which helps to placement the cells in order that a pointy, centered impression is attained.
Seem waves permit specific cell alignment, resulting in Excellent info high quality and ~10X speedier sample flow rates.
Application Take note: Automated image Evaluation reduces consumer-to-person variability in flow cytometry gating techniques
Leverage the power of Superior know-how and innovation to speed up your discoveries. Have confidence in from the Attune Flow Cytometer loved ones to deliver the reliability, speed, and accuracy that set your work aside.
No clean/no lyse leukocyte Evaluation with imaging. Cells ended up acquired from 24-hour-outdated blood by dilution in 1 mM EDTA ( Qualified UX/UI enables users to teach AI styles for expanded apps. (A) The “Prepare Design” characteristic delivers annotation resources and actual-time model education precision.
At the guts of each and every Attune Flow Cytometer lies our innovative acoustic focusing technological innovation, which utilizes sound waves to meticulously align cells in the flow cell. This ground breaking approach surpasses standard hydrodynamic techniques, leading to more rapidly processing speeds, Remarkable data quality, and improved sample throughput.
As the cell line experienced previously been analyzed around the Attune CytPix Flow Cytometer, the researchers went again to the images and were being in a position to doc the microbial infection at the least five times before.
The Attune CytPix Flow Cytometer enables consumers to visualise samples in the course of acquisition, confirm the precision of gating tactics, and assist effects with reliable documentation for publication uses.
What gave the impression to be a homogeneous populace is definitely a lot more diverse—an insight that ought to be regarded when interpreting benefits.